acf-free cell dissociation kit Search Results


sodium fluorophosphate  (Chem Impex International)
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acf-free cell dissociation kit  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc acf-free cell dissociation kit
Acf Free Cell Dissociation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/acf-free+cell+dissociation+kit/acf+cell+dissociation+kit/pmc06770901-40-5-9
Average 90 stars, based on 1 article reviews
acf-free cell dissociation kit - by Bioz Stars, 2026-09
90/100 stars
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collagenase a animal component-free (acf)  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc collagenase a animal component-free (acf)
Collagenase A Animal Component Free (Acf), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/acf-free+cell+dissociation+kit/collagenase+a+animal+component+free++acf+/us11279915-384-5-10
Average 90 stars, based on 1 article reviews
collagenase a animal component-free (acf) - by Bioz Stars, 2026-09
90/100 stars
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acf  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc acf
The NBVbe medium supports the human MSC isolation from the umbilical cords. a <t>Cell</t> isolation reagents comparison. Cell number was counted 30 days after the cell isolation. b Human MSCs were isolated with the different concentration of collagenase B. Cell number was counted 30 days after the cell isolation. c Human MSCs isolated from the umbilical cord, passage 0 and 6 cultured with the NBVbe medium. d Flow cytometry analysis of the human MSCs after isolated and cultured with the NBVbe medium. e Human MSCs were isolated and expanded with the NBVbe medium and then differentiated into adipocytes, osteocytes, and chondrocytes. Left: Oil Red O staining and anti- FABP4 immunostaining for the differentiated adipocytes; middle: Alizarin Red S staining and anti-Osteocalcin immunostaining for the differentiated osteocytes; right: Alcian blue staining and anti-Aggrecan immunostaining for the differentiated chondrocytes. Scale bar, 200 μm. An asterisk indicates P < 0.05. GCD, gentle cell <t>dissociation</t> reagent; SR, ReLeSR™; CDB, cell dissociation buffer, <t>enzyme-free;</t> ACF, <t>animal</t> <t>component-free</t> cell dissociation <t>kit</t>
Acf, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/acf-free+cell+dissociation+kit/acf/pmc07083066-84-30-35
Average 90 stars, based on 1 article reviews
acf - by Bioz Stars, 2026-09
90/100 stars
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animal component-free (acf) cell dissociation kit  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc animal component-free (acf) cell dissociation kit
The NBVbe medium supports the human MSC isolation from the umbilical cords. a <t>Cell</t> isolation reagents comparison. Cell number was counted 30 days after the cell isolation. b Human MSCs were isolated with the different concentration of collagenase B. Cell number was counted 30 days after the cell isolation. c Human MSCs isolated from the umbilical cord, passage 0 and 6 cultured with the NBVbe medium. d Flow cytometry analysis of the human MSCs after isolated and cultured with the NBVbe medium. e Human MSCs were isolated and expanded with the NBVbe medium and then differentiated into adipocytes, osteocytes, and chondrocytes. Left: Oil Red O staining and anti- FABP4 immunostaining for the differentiated adipocytes; middle: Alizarin Red S staining and anti-Osteocalcin immunostaining for the differentiated osteocytes; right: Alcian blue staining and anti-Aggrecan immunostaining for the differentiated chondrocytes. Scale bar, 200 μm. An asterisk indicates P < 0.05. GCD, gentle cell <t>dissociation</t> reagent; SR, ReLeSR™; CDB, cell dissociation buffer, <t>enzyme-free;</t> ACF, <t>animal</t> <t>component-free</t> cell dissociation <t>kit</t>
Animal Component Free (Acf) Cell Dissociation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/acf-free+cell+dissociation+kit/animal+component+free+cell+dissociation+kit/pm35538146-251-10-16
Average 90 stars, based on 1 article reviews
animal component-free (acf) cell dissociation kit - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

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The NBVbe medium supports the human MSC isolation from the umbilical cords. a Cell isolation reagents comparison. Cell number was counted 30 days after the cell isolation. b Human MSCs were isolated with the different concentration of collagenase B. Cell number was counted 30 days after the cell isolation. c Human MSCs isolated from the umbilical cord, passage 0 and 6 cultured with the NBVbe medium. d Flow cytometry analysis of the human MSCs after isolated and cultured with the NBVbe medium. e Human MSCs were isolated and expanded with the NBVbe medium and then differentiated into adipocytes, osteocytes, and chondrocytes. Left: Oil Red O staining and anti- FABP4 immunostaining for the differentiated adipocytes; middle: Alizarin Red S staining and anti-Osteocalcin immunostaining for the differentiated osteocytes; right: Alcian blue staining and anti-Aggrecan immunostaining for the differentiated chondrocytes. Scale bar, 200 μm. An asterisk indicates P < 0.05. GCD, gentle cell dissociation reagent; SR, ReLeSR™; CDB, cell dissociation buffer, enzyme-free; ACF, animal component-free cell dissociation kit

Journal: Stem Cell Research & Therapy

Article Title: Chemical-defined medium supporting the expansion of human mesenchymal stem cells

doi: 10.1186/s13287-020-01641-7

Figure Lengend Snippet: The NBVbe medium supports the human MSC isolation from the umbilical cords. a Cell isolation reagents comparison. Cell number was counted 30 days after the cell isolation. b Human MSCs were isolated with the different concentration of collagenase B. Cell number was counted 30 days after the cell isolation. c Human MSCs isolated from the umbilical cord, passage 0 and 6 cultured with the NBVbe medium. d Flow cytometry analysis of the human MSCs after isolated and cultured with the NBVbe medium. e Human MSCs were isolated and expanded with the NBVbe medium and then differentiated into adipocytes, osteocytes, and chondrocytes. Left: Oil Red O staining and anti- FABP4 immunostaining for the differentiated adipocytes; middle: Alizarin Red S staining and anti-Osteocalcin immunostaining for the differentiated osteocytes; right: Alcian blue staining and anti-Aggrecan immunostaining for the differentiated chondrocytes. Scale bar, 200 μm. An asterisk indicates P < 0.05. GCD, gentle cell dissociation reagent; SR, ReLeSR™; CDB, cell dissociation buffer, enzyme-free; ACF, animal component-free cell dissociation kit

Article Snippet: The dissociation reagents include TrypLE (Gibco), 0.05% Trypsin-EDTA (Gibco), Accutase (STEMCELL Technologies), GCD (Gentle Cell Dissociation Reagent, STEMCELL Technologies), SR (ReLeSR™, STEMCELL Technologies), CDB (Cell Dissociation Buffer, enzyme-free, Gibco), ACF (Animal Component-Free Cell Dissociation Kit, STEMCELL Technologies), Collagenase B (STEMCELL Technologies, diluted in DMEM/High Glucose), and DNase I (Sigma), Dispase II (Sigma).

Techniques: Isolation, Cell Isolation, Concentration Assay, Cell Culture, Flow Cytometry, Staining, Immunostaining